|
Developmental Studies Hybridoma Bank
alkaline phosphatase alp Alkaline Phosphatase Alp, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/alkaline phosphatase alp/product/Developmental Studies Hybridoma Bank Average 94 stars, based on 1 article reviews
alkaline phosphatase alp - by Bioz Stars,
2026-03
94/100 stars
|
Buy from Supplier |
|
TaKaRa
tks gflextm dna polymerase Tks Gflextm Dna Polymerase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/tks gflextm dna polymerase/product/TaKaRa Average 98 stars, based on 1 article reviews
tks gflextm dna polymerase - by Bioz Stars,
2026-03
98/100 stars
|
Buy from Supplier |
|
Addgene inc
5.4 kb mouse ap2 promoter construct 5.4 Kb Mouse Ap2 Promoter Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/5.4 kb mouse ap2 promoter construct/product/Addgene inc Average 90 stars, based on 1 article reviews
5.4 kb mouse ap2 promoter construct - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
Addgene inc
ap2-angptl4 tg rats (adipose tissue specific) Ap2 Angptl4 Tg Rats (Adipose Tissue Specific), supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/ap2-angptl4 tg rats (adipose tissue specific)/product/Addgene inc Average 90 stars, based on 1 article reviews
ap2-angptl4 tg rats (adipose tissue specific) - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
Promega
pgl3-basic Pgl3 Basic, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/pgl3-basic/product/Promega Average 90 stars, based on 1 article reviews
pgl3-basic - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
Jackson Laboratory
transgenic mouse Transgenic Mouse, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/transgenic mouse/product/Jackson Laboratory Average 90 stars, based on 1 article reviews
transgenic mouse - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
Thermo Fisher
pcr-blunt ii topo Pcr Blunt Ii Topo, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/pcr-blunt ii topo/product/Thermo Fisher Average 90 stars, based on 1 article reviews
pcr-blunt ii topo - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
Jackson Laboratory
c57bl/6j mice wild-type (wt) mice C57bl/6j Mice Wild Type (Wt) Mice, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/c57bl/6j mice wild-type (wt) mice/product/Jackson Laboratory Average 90 stars, based on 1 article reviews
c57bl/6j mice wild-type (wt) mice - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
Developmental Studies Hybridoma Bank
anti-rhob 54.4h7 Anti Rhob 54.4h7, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/anti-rhob 54.4h7/product/Developmental Studies Hybridoma Bank Average 90 stars, based on 1 article reviews
anti-rhob 54.4h7 - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
Thermo Fisher
ta cloning-vector Ta Cloning Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/ta cloning-vector/product/Thermo Fisher Average 90 stars, based on 1 article reviews
ta cloning-vector - by Bioz Stars,
2026-03
90/100 stars
|
Buy from Supplier |
|
TaKaRa
embryo cdna Embryo Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/embryo cdna/product/TaKaRa Average 93 stars, based on 1 article reviews
embryo cdna - by Bioz Stars,
2026-03
93/100 stars
|
Buy from Supplier |
|
Abcam
alg5  Alg5, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/result/alg5/product/Abcam Average 98 stars, based on 1 article reviews
alg5 - by Bioz Stars,
2026-03
98/100 stars
|
Buy from Supplier |
Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis
doi: 10.3892/etm.2017.5444
Figure Lengend Snippet: Primer sequences for specific genes.
Article Snippet: Total 50 µg protein sample (per lane) was separated on SDS-PAGE gel, blotted onto PVDF membranes, and blocked in 5% non-fat milk for 1 h. The membranes were probed with mouse polyclonal antibodies to ULK1 (120 kDa; 1;1,000; ab128859; Abcam, Cambridge, UK),
Techniques: Sequencing
Journal: Experimental and Therapeutic Medicine
Article Title: miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis
doi: 10.3892/etm.2017.5444
Figure Lengend Snippet: TNF-α treatment significantly decreases cell viability and autophagy, as well as increases apoptosis and the expression of miR-4262 in chondrocytes. (A) The cell viability in control and TNF-α groups at 0, 12, 24, 48 h using CCK-8; (B) The cell apoptosis in control and TNF-α groups at 0, 12, 24, 48 h using TUNEL staining; (C) The expression of autophagy-related proteins, including ULK1, ALG5, Beclin-1, LC3II/LC3I, in control, TNF-α, RAPA and TNF-α + E64d + pepstatin A groups using western blotting; (D) The level of miR-4262 in control and TNF-α groups at 0, 12, 24, 48 h using quantitative reverse transcription PCR. Three independent experiments were performed in each assay. *P<0.05, **P<0.01, and ***P<0.001 compared with the control group, #P<0.05 compared with the TNF-α group. TNF-α, tumor necrosis factor-α; RAPA, rapamycin; ULK1, uncoordinated 51-like kinase 1; ALG5, asparagine-linked glycosylation 5.
Article Snippet: Total 50 µg protein sample (per lane) was separated on SDS-PAGE gel, blotted onto PVDF membranes, and blocked in 5% non-fat milk for 1 h. The membranes were probed with mouse polyclonal antibodies to ULK1 (120 kDa; 1;1,000; ab128859; Abcam, Cambridge, UK),
Techniques: Expressing, CCK-8 Assay, TUNEL Assay, Staining, Western Blot
Journal: Experimental and Therapeutic Medicine
Article Title: miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis
doi: 10.3892/etm.2017.5444
Figure Lengend Snippet: Upregulated miR-4262 further decreases cell viability, autophagy, and matrix synthesis as well as increases apoptosis in TNF-α-treated chondrocytes. (A) The miR-4262 level in control, scramble (control of mimic), miR-4262 mimic, NC (control of inhibitor), and miR-4262 inhibitor groups by qPCR; (B) The cell viability in control, scramble, miR-4262 mimic, inhibitor NC, and miR-4262 inhibitor groups using CCK-8; (C) The cell apoptosis in control, scramble, miR-4262 mimic, inhibitor NC, and miR-4262 inhibitor groups using TUNEL staining; (D) The expression of autophagy-related proteins, including ULK1, ALG5, Beclin-1, LC3II/LC3I, in control, scramble, miR-4262 mimic, inhibitor NC, and miR-4262 inhibitor groups using western blotting; (E) The cell viability in control, TNF-α, TNF-α + scramble, TNF-α + miR-4262 mimic, TNF-α + NC, and TNF-α + miR-4262 inhibitor groups using CCK-8; (F) The cell apoptosis in control, TNF-α, TNF-α + scramble, TNF-α + miR-4262 mimic, TNF-α + NC, and TNF-α + miR-4262 inhibitor groups using TUNEL staining; (G) The expression of autophagy-related proteins, including ULK1, ALG5, Beclin-1, LC3II/LC3I, in control, TNF-α, TNF-α+E64d+pepstatin (A) TNF-α + scramble, TNF-α + miR-4262 mimic, TNF-α + NC, and TNF-α + miR-4262 inhibitor groups using western blotting; (H) The expression of matrix synthesis-related proteins, such as COL2A1, ACAN, MMP-13 and ADAMTS-5, in control, TNF-α, TNF-α + scramble, TNF-α + miR-4262 mimic, TNF-α + NC, and TNF-α + miR-4262 inhibitor groups using western blotting. Three independent experiments were performed in each assay. *P<0.05, **P<0.01, and ***P<0.001 TNF-α, tumor necrosis factor-α; ULK1, uncoordinated 51-like kinase 1; ALG5, asparagine-linked glycosylation 5; COL2A1, type II collagen; ACAN, aggrecan; MMP-13, matrix metallo protease 13; ADAMTS-5, a disintegrin and metalloproteinase with thrombospondin motifs-5.
Article Snippet: Total 50 µg protein sample (per lane) was separated on SDS-PAGE gel, blotted onto PVDF membranes, and blocked in 5% non-fat milk for 1 h. The membranes were probed with mouse polyclonal antibodies to ULK1 (120 kDa; 1;1,000; ab128859; Abcam, Cambridge, UK),
Techniques: CCK-8 Assay, TUNEL Assay, Staining, Expressing, Western Blot
Journal: Experimental and Therapeutic Medicine
Article Title: miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis
doi: 10.3892/etm.2017.5444
Figure Lengend Snippet: Upregulated SIRT1 increases cell viability, autophagy, and matrix synthesis as well as decreases apoptosis in TNF-α-treated chondrocytes. (A) The SIRT1 level in control, pEX, pEX-SIRT1, siNC, and si-SIRT1 groups by qPCR; (B) The SIRT1 level in control, pEX, pEX-SIRT1, siNC, and si-SIRT1 groups by western blotting; The cell viability in (C) control, TNF-α, TNF-α + pEX, TNF-α + pEX-SIRT1, TNF-α + siNC, and TNF-α + si-SIRT1 groups using CCK-8; (D) The cell apoptosis in control, TNF-α, TNF-α + pEX, TNF-α + pEX-SIRT1, TNF-α + siNC, and TNF-α + si-SIRT1 groups using TUNEL staining; (E) The expression of autophagy-related proteins, including ULK1, ALG5, Beclin-1, LC3II/LC3I, in control, TNF-α, TNF-α + pEX, TNF-α + pEX-SIRT1, TNF-α + siNC, and TNF-α + si-SIRT1 groups using qPCR; (F) The expression of matrix synthesis-related proteins, such as COL2A1, ACAN, MMP-13 and ADAMTS-5, in control, TNF-α, TNF-α + pEX, TNF-α + pEX-SIRT1, TNF-α + siNC, and TNF-α + si-SIRT1 groups using qPCR. Three independent experiments were performed in each assay. *P<0.05, and **P<0.01 TNF-α, tumor necrosis factor-α; ULK1, uncoordinated 51-like kinase 1; ALG5, asparagine-linked glycosylation 5; COL2A1, type II collagen; ACAN, aggrecan; MMP-13, matrix metallo protease 13; ADAMTS-5, a disintegrin and metalloproteinase with thrombospondin motifs-5; SIRT1, sirtuin type 1.
Article Snippet: Total 50 µg protein sample (per lane) was separated on SDS-PAGE gel, blotted onto PVDF membranes, and blocked in 5% non-fat milk for 1 h. The membranes were probed with mouse polyclonal antibodies to ULK1 (120 kDa; 1;1,000; ab128859; Abcam, Cambridge, UK),
Techniques: Western Blot, CCK-8 Assay, TUNEL Assay, Staining, Expressing
Journal: Experimental and Therapeutic Medicine
Article Title: miR-4262 regulates chondrocyte viability, apoptosis, autophagy by targeting SIRT1 and activating PI3K/AKT/mTOR signaling pathway in rats with osteoarthritis
doi: 10.3892/etm.2017.5444
Figure Lengend Snippet: Effects of miR-4262 on cell viability, cell apoptosis, cell autophagy, and matrix synthesis is inhibited by SIRT1. (A) The cell viability in control, TNF-α, TNF-α + scramble + pEX, TNF-α + miR-4262 mimic, and TNF-α + miR-4262 mimic + pEX-SIRT1 groups using CCK-8; (B) The cell apoptosis in control, TNF-α, TNF-α + scramble + pEX, TNF-α + miR-4262 mimic, and TNF-α + miR-4262 mimic + pEX-SIRT1 groups using TUNEL staining; (C) The expression of autophagy-related proteins, including ULK1, ALG5, Beclin-1, LC3II/LC3I, in control, TNF-α, TNF-α + scramble + pEX, TNF-α + miR-4262 mimic, and TNF-α + miR-4262 mimic + pEX-SIRT1 groups using western blotting; (D) The expression of matrix synthesis-related proteins, such as COL2A1, ACAN, MMP-13 and ADAMTS-5, in control, TNF-α, TNF-α + scramble + pEX, TNF-α + miR-4262 mimic, and TNF-α + miR-4262 mimic + pEX-SIRT1 groups using western blotting. Three independent experiments were performed in each assay. *P<0.05, and **P<0.01 TNF-α, tumor necrosis factor-α; ULK1, uncoordinated 51-like kinase 1; ALG5, asparagine-linked glycosylation 5; COL2A1, type II collagen; ACAN, aggrecan; MMP-13, matrix metallo protease 13; ADAMTS-5, a disintegrin and metalloproteinase with thrombospondin motifs-5; SIRT1, sirtuin type 1.
Article Snippet: Total 50 µg protein sample (per lane) was separated on SDS-PAGE gel, blotted onto PVDF membranes, and blocked in 5% non-fat milk for 1 h. The membranes were probed with mouse polyclonal antibodies to ULK1 (120 kDa; 1;1,000; ab128859; Abcam, Cambridge, UK),
Techniques: CCK-8 Assay, TUNEL Assay, Staining, Expressing, Western Blot